Wykaz obszarów badawczych związanych z tagiem LC-MS:
| # | Obszar badawczy | Dziedzina naukowa |
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| 1 |
Mass spectrometry combined with liquid chromatography-mass spectrometry (LC-MS) is increasingly used in routine medical diagnostics. It happened because LC-MS methods for determining markers of metabolic disorders provide unique sensitivity and selectivity. However, a significant disadvantage of this technique is the need to deliver body fluids to the analytical laboratory. As a result, methods are being developed to determine metabolites in a dry blood sample (DBS). A drop of blood can be applied to blotting paper, dried by the patient, and then sent to the laboratory. The determination of metabolites by LC-MS can be automated and leads to a significant reduction in analysis costs. The work carried out in this area requires the development of methods to determine metabolite groups specific to the selected disease. During the work, it is necessary to consider the stability and degree of homogeneity of tested samples to obtain a sensitive and reproducible analytical method.
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| 2 |
Liposomes are nanosized vesicles built from a phospholipid double layer. Currently, intensive works are carried out on the broad application of this type of nanomaterials in more effective delivery of active compounds, e.g., in dietary supplements. Unfortunately, it is a lack of studies confirming the increased bioavailability of active compounds from liposomal preparations than from classic ones. Therefore, research aimed at understanding their metabolic pathways (and comparing them with supplements in which nanomaterials are not used) in conditions that simulate the human body is a significant issue. As part of the dissertation, the changes mentioned above concerning selected supplements, e.g., zinc or vitamin B12, will be tested using, on the one hand, modern methods of in vitro simulation (enzymatic digestion/degree of absorption into intestinal villus cell lines), and on the other hand, high-sensitive mass spectrometry detection techniques.
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| 3 |
Metal-based nanoparticles are increasingly being considered as fertilizer additives, enabling more effective plant growth and ensuring their greater resistance to diseases. Unfortunately, despite great interest in introducing this type of nanostructures into circulation, there is a lack of research focused on understanding their metabolic pathways in the human body and the forms in which they are absorbed by the human body. As part of the dissertation, analytical methods will be developed, using mass spectrometry detection techniques - both molecular and atomic, thanks to which the transformations of selected metal nanoparticles will be examined in vitro - from simulating the digestive processes of selected edible farmed plants to identifying the forms of metals absorbed by intestinal cilia cell lines
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