Wykaz obszarów badawczych związanych z tagiem Egzosomy:
| # | Obszar badawczy | Dziedzina naukowa |
|---|---|---|
| 1 |
Exosomes are extracellular signaling molecules secreted by eukaryotic cells, the presence of which in body fluids makes them a potential and easily accessible biomarkers of various pathological processes, including cancer. Taking blood serum rich in exosomes from patients with cancer is a simple and minimally invasive procedure. Methods for detecting exosomes can be used not only to monitor the stage of a given tumor, but also to assess the effectiveness of the applied therapy, including immunotherapy, targeted or combined therapy. The aim of the project is to optimize a specific method of isolation and determination of the content exosomes in blood samples from patients in order to monitor the therapy of cancer.The project is implemented in cooperation with Charité - Universitätsmedizin Berlin as part of the "Institutpartnerschaft" program granted by Alexander von Humboldt foundation.
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| 2 |
My research focuses on determining the anticancer potential of various active compounds. In particular, the research is concerned with determining the cytotoxicity of potential drugs against various cancer and normal lines, determining the type of cell death (apoptosis, autophagy) and activation of selected signaling pathways in the cell. Most of the ongoing research involves inhibitors of protein kinases CK2 and PIM and their use in leukemia and breast cancer therapy. Ongoing research also includes determining synergistic interactions of selected protein kinase inhibitors together with classical cytostatics. Recent research includes the use of human exosomes to improve the efficacy of selected CK2 inhibitors on cancer cells. Methods he specializes in include MTT assay (determination of IC50 values, combination indices (CI), dose reduction index (DRI)), flow cytometry methods, immunodetection (western blot), immunofluorescence (fluorescence, confocal microscopy).
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